流式细胞多色分析优秀课件.ppt

《流式细胞多色分析优秀课件.ppt》由会员分享，可在线阅读，更多相关《流式细胞多色分析优秀课件.ppt（10页珍藏版）》请在得力文库 - 分享文档赚钱的网站上搜索。

1、流式细胞多色分析流式细胞多色分析23-13536-00 Rev.01第1页，本讲稿共10页Multicolor Analysis StrategiesPlot typesHierarchical gatingPopulation hierarchiesBack gating223-13536-00 Rev.01第2页，本讲稿共10页Use Different Plot TypesHistogramsusing histograms can help discriminate between two populations.Dot Plot:hard to discriminate popula

2、tionsDot Plot:displaying fewer events improves discriminationHistogram:population boundary is evident 323-13536-00 Rev.01第3页，本讲稿共10页Use Different Plot TypesContour Plotsusing contour plots can:Help locate population means.Indicate a need for biexponential scaling.Dot Plot:hard to see populations bun

3、ched on axisContour Plot:shows that biexponential display might be needed Biexponential Contour Plot:gives best display of populations 423-13536-00 Rev.01第4页，本讲稿共10页Hierarchical Gating523-13536-00 Rev.01第5页，本讲稿共10页Hierarchical GatingWhat went wrong here?Correct Hierarchy623-13536-00 Rev.01第6页，本讲稿共10

4、页Back GatingResearcher wants to discriminate Tregs based on CD25 and CD127 expression.Need to ensure a gate includes the entire FOXP3+population.723-13536-00 Rev.01第7页，本讲稿共10页Additional Analysis StrategiesBiexponential scalingEvents to displayHinged and offset quadrant gatesDerived(boolean)gatesSnap

5、-to gatesStatisticsAnalysis templates823-13536-00 Rev.01第8页，本讲稿共10页ICS Data Analysis ResultsInformation that can be retrieved:Number of collected events,number of live CD3+cells%of main populations:%CD3+,CD4+,CD8+%of nave(CD45RA+)and memory(CD45RA)CD4+and CD8+cells%CD25+CD4+cells(Tregs)For the cytokines:%of CD4+and CD8+making 1 or 2 cytokinesAnalysis Exercise923-13536-00 Rev.01第9页，本讲稿共10页ICS Panel Analysis1023-13536-00 Rev.01第10页，本讲稿共10页