流式细胞多色分析优秀PPT.ppt

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1、流式细胞多色分析流式细胞多色分析23-13536-00 Rev.01你现在浏览的是第一页，共10页Multicolor Analysis StrategiesPlot typesHierarchical gatingPopulation hierarchiesBack gating223-13536-00 Rev.01你现在浏览的是第二页，共10页Use Different Plot TypesHistogramsusing histograms can help discriminate between two populations.Dot Plot:hard to discriminat

2、e populationsDot Plot:displaying fewer events improves discriminationHistogram:population boundary is evident 323-13536-00 Rev.01你现在浏览的是第三页，共10页Use Different Plot TypesContour Plotsusing contour plots can:Help locate population means.Indicate a need for biexponential scaling.Dot Plot:hard to see pop

3、ulations bunched on axisContour Plot:shows that biexponential display might be needed Biexponential Contour Plot:gives best display of populations 423-13536-00 Rev.01你现在浏览的是第四页，共10页Hierarchical Gating523-13536-00 Rev.01你现在浏览的是第五页，共10页Hierarchical GatingWhat went wrong here?Correct Hierarchy623-13536

4、-00 Rev.01你现在浏览的是第六页，共10页Back GatingResearcher wants to discriminate Tregs based on CD25 and CD127 expression.Need to ensure a gate includes the entire FOXP3+population.723-13536-00 Rev.01你现在浏览的是第七页，共10页Additional Analysis StrategiesBiexponential scalingEvents to displayHinged and offset quadrant ga

5、tesDerived(boolean)gatesSnap-to gatesStatisticsAnalysis templates823-13536-00 Rev.01你现在浏览的是第八页，共10页ICS Data Analysis ResultsInformation that can be retrieved:Number of collected events,number of live CD3+cells%of main populations:%CD3+,CD4+,CD8+%of nave(CD45RA+)and memory(CD45RA)CD4+and CD8+cells%CD25+CD4+cells(Tregs)For the cytokines:%of CD4+and CD8+making 1 or 2 cytokinesAnalysis Exercise923-13536-00 Rev.01你现在浏览的是第九页，共10页ICS Panel Analysis1023-13536-00 Rev.01你现在浏览的是第十页，共10页