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1、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

2、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

3、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

4、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

5、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

6、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

7、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

8、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

9、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

10、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

11、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

12、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

13、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

14、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

15、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

16、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

17、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

18、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

19、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

20、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

21、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

22、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

23、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

24、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

25、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

26、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

27、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

28、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

29、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

30、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

31、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

32、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

33、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

34、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

35、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

36、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

37、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不

38、不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不不(装订线内不要答题)装订线20192020 学年度下学期期中考试高二试题生物参考答案与评分标准一、 选择题 (每题1.5分, 共54分, 每题只

39、有一个正确选项)1-5CDCCD6-10BDCCB11-15DDDCD16-20DCCAD21-25CAABB26-30BDCAB31-36DAADDB二、 非选择题 (46分)37. (14分, 每空1分)(1) 基因工程 (转基因技术)植物细胞工程 (植物组织培养)(2) 基因表达载体的构建Ti质粒上的T-DNA可转移至受体细胞, 并且整合到受体细胞染色体DNA上(3) 脱分化细胞分裂素生长素卡那霉素分子杂交转录杂交带(4) PCR (多聚酶链式反应)Taq酶 (热稳定DNA聚合酶)2n+1-238. (11分, 除标注外每空1分)(1) 供体体细胞、 供体卵母细胞、 重组细胞 (写出两个

40、即可得分, 其他答案合理也可以得分)(2分)无菌、 无毒 (全对得分)抗生素(2) 保持正常二倍体核型促性腺激素第一极体显微操作去核法 ( “去核法” 这三个字可以不答)透明带和卵母细胞质膜 (或答卵母细胞膜、 卵细胞膜, 两个都对才得分)(3) 体细胞分化程度高, 恢复其全能性十分困难 (合理即可)遗传物质相同39. (8分, 每空1分)(1) 细胞核移植、 早期胚胎培养(2) 内细胞团(3) 分化诱导因子发育的全能性(4) 显微注射分泌胰岛素体外基因治疗40. (13分, 除标注外每空1分)(1) 杀菌、 浓缩麦汁、 破坏酶的活性、 排除麦汁中特异杂臭气体等作用 (其他答案合理也可给分, 2分)淀粉酶等多种酶 (答出淀粉酶就得分)(2) C2H5OH+O2CH3COOH+H2O(3) 乳酸菌在无氧的环境下大量繁殖, 将葡萄糖分解成乳酸盐酸酸化重氮化N-1一萘基乙二胺盐酸盐(4) 减慢pH不适宜 (pH过低)(5)乳酸菌乳酸亚硝酸盐含量OOO发酵时间发酵时间发酵时间乳酸菌的曲线, 前半阶段画出 “S” 形, 后半段下降即可乳酸的曲线有上升趋势即可高二生物(答)1

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