实验7.11药物LD50和ED50的测定17.ppt

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1、实验实验7.117.11药物药物LDLD5050和和EDED5050的测定的测定【目的】通过测定戊巴比妥钠的LD50和ED50来评价其平安性。【原理】1.戊巴比妥钠为镇静催眠药，随着剂量加大，相继出现镇静、催眠、抗惊厥、麻醉、死亡。2.药物效应和药物剂量的关系称量效关系，从不同角度观察，有量反响和质反响之分。量反响：特定反响程度与剂量的关系，如血压的升高、血糖的降低等。质反响：特定反响出现与否与剂量的关系，如死亡与否、阳性或阴性等。3.质反响实验中，以对数剂量为横坐标、反响百分率为纵坐标绘图，形成一条对称的“S形曲线。半数有效量ED50：50的实验动物有效时对应的剂量。半数致死量LD50：50

2、的实验动物死亡时对应的剂量。4.药物平安性的评价 治疗指数TI=LD50/ED50 平安系数SF LD5/ED95 可靠平安系数CSF LD1/ED99 三者数值大，说明药物平安性好。质反响的量效曲线睡眠死亡反响百分率020408060100剂量LD50ED505.实验设计三大原那么：重复、随机、对照 随机原那么：使每个实验对象在接受处理因素时，有相同的时机，以减少主观因素所带来的偏性误差。均衡随机法分组：先按易控制的因素性别、年龄、体重等均衡分档，然后在每一档随机取出等量的动物分配到各组，使难控制的因素活泼程度、饥饱、疲劳程度等得到随机安排。均衡随机分组法均衡随机分组法【器材与药品】小鼠笼、

3、1ml注射器1副、天平秤、0.4戊巴比妥钠溶液。【实验动物】小白鼠、体重18-22g，雌雄各半。实验前禁食12h，不禁水。【实验步骤】1、随机分组：5个剂量组，每组各10只。2、称重、标记。3、各组分别腹腔注射戊巴比妥钠 20、25、31、39、49mg/kg0.2ml/10g；以翻正反射消失为入睡指标，给药15分钟后，记录各组睡眠鼠数填入表中。4、计算机Bliss法算出：LD50、LD1、LD5、ED50、ED99、ED95。5.计算TI、SF、CSF。剂量 实验鼠数 睡眠鼠数 反响百分率 ED50mg/kg mg/kg 49 10 39 10 31 10 25 10 20 10 不同剂量戊

4、巴比妥钠对小鼠睡眠的影响【本卷须知】1.分组时采用均衡随机法。2.小鼠入睡指标翻正反射消失，保持室内安静，已睡眠鼠与未睡眠鼠分笼放置。3.绘图时注意标明坐标、曲线方程及曲线名称。【讨论】如何评价药物的平安性？Xp#s%v)y0C3F6IaLdOgSjVnYq!t*w-z1D4G8JbMeQhTkWoZr%u(x+B2E5H9KcOfRiUmXp#s&v)z0C3F7IaLdPgSjVnYq$t*w-A1D4G8JbNeQhTlWoZr%u(y+B2E6H9KcOfRjUmXp!s&v)z0C4F7IaMdPgSkVnZq$t*x-A1D5G8KbNeQiTlWo#r%u(y+B3E6H9LcO

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