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1、SODPODCATMDAAPXA0.2MKH2P04KH2P0427.2161000 B 0.2M K2HP04K2HP04 3H20 45.644 1000 A 0.2M NaH2P04 NaH2P04*2H20 31 21 1000 B 0.2M Na2HP04Na2HP04 12H20 71.64 1000 05g5PH=7.820(10000 Xg),04 CSOD1.SOD 1)05M(PH7.8):A21.25mI+B228.25mI 1000ml;(2)130mM Met 1.9399Met 100ml;(3)750 RM(NBT):0.06133gNBT 100ml(4)100
2、 uM EDTA-Na2:0.0372g EDTA-Na2 1000ml;(5)20 u MFD 0.00753gFD 1000ml S0DMet NBT EDTA-Na2(FD)H20 15 3 3 3 3 2.5,2.SOD 2034000Lux30(CK)4000Lux30560nm3.SOD/gFW)=(ACKAE)XV/(WX0.5 X ACK)NBT50%SOD/mgSODPOD 1.0.1MPH6.0A21925+B30.75,500 2.POD0.1M pH 6.0502830%H202193.POD20+3470nm1(2470/0)*mg pr AA470/mgFW)4.P
3、OD(A470/min gFW)=AA470X V/Va/W=AA470X5/0.02/0.5=A A470 X 500 CAT 1.CAT0.1MH202:056830%H202100 0.1MPH7.097.5A+152.5B 500 0025+0.1MPH7.0204CAT 2.CAT0.150+2.52401133CAT A240/min gFW)=A 240 X/0 05/0.5=A 240X200=A 240XV/Va/W MDA 1.MDA0.6TBA 1 MNaOH10%TCA 1003.MDA1+20.6%TBA,15600532450nm4.MDA(u mo I/gfw)=
4、(6.45X g-DQ-0.56DQ X0.015/W(6.45 X(D532-D6OO)-0.56D450)X 0.03/W 0.1G-2505090%10085%100020+3G-2502595(20+3G-250)(mg/Gfw)=(CXV/Va)/W 06200):3.75+90+36 0.35102223405520(ug/g=CXV/Va/W APX(ASA-P0D)05g,5ml 4 C12000g-min-1 20min,APX4 C0.186g EDTA,0.4718g ASA,pH7.0500ml,APXAPX 11.2 L30%H202+0.04718g AsA(Vc),(pH7.0)500ml 0.1ml+2.9mI APX290nm(0-40s)o