兔单克隆抗体了解兔单克隆抗体的精彩课件.ppt

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1、Rabbit Monoclonal Antibodies-Better Tools for Better Science2023/2/71 Advantages of RabMAb Technology Over Mouse MAb TechnologyHigher quality High affinity and specificityBroad applications High success rate in meeting customers criteriaNovel Epitopes Recognition of mouse orthologs with high homolog

2、y to human antigensHigher throughputStable hybridomasMultiplexHigh humanization probabilityHigher homology to human germline sequence than mouseA in vitro positive proof-of-concept study2023/2/7Rabbits Recognize More EpitopesMouseRabbit1.Molecular-weight marker2.Fibronectin3.Protease digested fibron

3、ectin1 2 3 1 2 35 mice and 3 rabbitsWestern AnalysisPolyseraImmunized with human fibronectin2023/2/7Supernatant From Rabbit Hybridoma Is Highly Active In Western blotABCD(A=1:5;B=1:50;C=1:500;D=1:1000)K8Anti-Human Keratin 8 MAb2023/2/7A Random Collection Of RabMAbs Have Extremely High AffinitiesAnti

4、gen MAbKon(1/M.sec)Koff(1/sec)Kd(M)EREPIer392.58x1052.45x10-39.5x10-9EREPIer142.03x1059.28x10-54.57x10-10EREPIer261.72x1053.37x10-52.19x10-10EREPIer382.87x1057.79x10-42.71x10-9PREPIpr421.18x1061.63x10-21.38x10-8PREPIpr333.22x1064.13x10-61.28x10-12PREPIpr884.46x1052.51x10-45.63x10-10C29EPIc29027.61x1

5、042.88x10-33.78x10-8C29EPIc29033.26x1043.12x10-69.57x10-11C29EPIc29051.36x1054.96x10-43.65x10-9C29EPIc29063.43x1044.57x10-51.33x10-9HerceptinKd(M)=5.0 x10-9$300MRituxan8.0 x10-9$1.1BSynagis1.0 x10-9$600MRemicade2.0 x10-10$270M2023/2/7RabMAb For Better DiagnosticsMouse 1A6RabMAbHeat treateduntreated2

6、023/2/7Rabbit MAbMouse MAbPhage MAbNon Primary AbRabMAb:More Accurate Results-Comparison of rabbit MAb,mouse MAb and phage MAb50%false positive15%false positive5%false positive2023/2/7Fibrin Degradation ProductsFibrin(Blood clot)PlasminPlasminogenEndothelia CellPAI-1tPAPropertyApplicationtPA RabMAbc

7、lone8BindDiagnosticsclone15InhibitTherapeuticsclone22StimulateTherapeuticsclone29StrongstimulateTherapeuticsclone34BindDiagnosticsPAI-1 RabMAbclone2InhibitPAI-1/tPATherapeuticsclone7BindDiagnosticsclone10InhibitPAI-1/tPATherapeuticsclone12InhibitPAI-1/tPATherapeuticsclone14BindDiagnosticsOne Process

8、:Multiple RabMAb ProductsPAI-1tPA2023/2/7PathoMAb to Identify Therapeutic MAb and Disease TargetTumor cellEmbryonic cellInflammatory tissueDegenerative tissueMembrane fractionNuclear fractiongenerates up to 10,000 MAbs from disease tissues directly YYYYYAntigensBioactivityTherapeutic MAbcandidatesDi

9、seasetargetsCell-based assays2023/2/7MAbs Against Mouse Mammary Tumors Raised From A Single Rabbit7000hybridomaclonesgenerated:157of1152antibodiesareusefulforimmunohistochemistry80antibodiespreferentiallyrecognizetumor57antibodiesreactwithepitheliumcells2antibodiesbindspecificfornecroticareasoftumor

10、11antibodiesreactwithstroma2antibodies staincell-celljunctions 5antibodies detectsecretedmolecules10antibodiesreactwithmyoepithelialcells2023/2/7Blocking MAbs for Tumor Cell Adhesion2023/2/7Primary tumor(20%expression)Lung metastasis(100%expression)RabMAb To A Novel Ion Channel Tumor Antigen-identif

11、ied directly from immunization of cancer tissues2023/2/7Using Subtractive PathoMAb to Identify MAbs Specific to Disease TissuesImmunize Rabbit withLung Cancer CellsScreen Hybridoma Against Lung Cancer Cells Back-Screen Positives Against Normal Lung Cells 100 MAbs5 MAbs2023/2/7A Kidney Specific Antib

12、ody Generated by PathoMAbBrainLungLiverKidney2023/2/7Proof Of Principle For Humanized RabMAb-from 75%to 95%human sequenceHzdMAbRabMAb293 b b-6 transfectants293 untransfected2023/2/7Market Demand For RabMAbsSmallMoleculesPeptidesMTMProteinsMouseMAbsUnavailable-NovelResearchreagentsorleadsforresearch,

13、drugdiscoveryanddiagnostics.MouseMAbsAvailable-Opportunityforbetterqualityfollow-onantibodiesHigherAffinityDiagnosticsNovelEpitopesHigherAffinitiesTherapeuticsAntibodyPairsResearch ReagentAntibodyArrays2023/2/7Improve The Probability Of Success For Drug DiscoveryRabMAbTechnologyTarget ID&ValidationP

14、rotein level,Clinical-directedtarget validationMultiplex,Companion diagnosticsNovel Epitope,High affinityTherapeutic leadsDiagnostic leads2023/2/7Success Rate Of RabMAb Generation2023/2/7CustomersCustomersCustomers2023/2/7SummaryRabMAbisasuperiortechnologyplatformthatiswidelyacceptedbytheindustryEpitomicsenjoysrapidgrowthincustomersforresearch,diagnosticandtherapeuticfieldsRabbitMAbshavehighaffinity;Rabbithybridomasarestable;Thesuccessratetoobtain“desirable”MAbsishigh;Thethroughputcanbehigh.2023/2/7