各种缓冲液的配制方法大全2.pdf

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1、磷酸氢二钠 柠檬酸缓冲液Na2HPO4 分子量=14.98，0.2mol/L 溶液为 28.40 克/升。Na2HPO4 2H2O 分子量=178.05，0.2mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14，0.1mol/L 溶液为 21.01 克/升20%盐酸溶液如何配置：取浓盐酸（质量百分比浓度为36.5%）一个体积（如 100 毫升），加入到 96.5毫升 水 中 就 可 以 了。36.5%*100*1.17=20%*mm=213.5(克)所 需 水 的 质 量为;213.5-117=96.5克，也就是 96.5毫升水。PH 0.2mol/L Na2H

2、PO4（毫升）0.1mol/L 柠檬酸（毫升）pH 0.2mol/L Na2HPO4（毫升）0.1mol/L 柠檬酸（毫升）2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 0.40 1.24 2.18 3.17 4.11 4.94 5.70 6.44 7.10 7.71 8.28 8.82 9.35 9.86 10.30 10.60 18.76 17.82 16.83 15.89 15.06 14.30 13.56 12.90 12.29 11.72 11.18 10.65 10.14 9.70 5.2 5.4 5.6 5

3、.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0 10.72 11.15 11.60 12.09 12.63 13.22 13.85 14.55 15.45 16.47 17.39 18.17 18.73 19.15 19.45 9.28 8.85 8.40 7.91 7.37 6.78 6.15 5.45 4.55 3.53 2.61 1.83 1.27 0.85 0.55 磷酸盐缓冲液配制方法（PB）磷酸盐缓冲液（Phosphate Buffer,PB）试剂：NaH2PO4 2H2O Na2HPO4 12H2O 配制方法：配制时，常先配制0.2m

4、ol/L的 NaH2PO4和 0.2mol/L的 Na2HPO4，两者按一定比例混合即成 0.2mol/L的磷酸盐缓冲液（PB），根据需要可配制不同浓度的PB 和 PBS。（1）0.2mol/L的 NaH2PO4；称取 NaH2PO4.2H2O 31.2g(或 NaH2PO4 H2O 27.6g)加重蒸水至 1000ml溶解。（2）0.2mol/L的 Na2HPO4：称取 Na2HPO4.12H2O 71.632g（或 Na2HPO4 7H2O 53.6g或 Na2HPO4 2H2O 35.6g）加重蒸水至1000ml溶解。（3）0.2mol/L pH7.4的 PB 的配制：取19ml 0.2

5、mol/L的 NaH2PO4和 81ml 0.2mol/L的Na2HPO4 12H2O,充分混合即为0.2mol/L的 PB（pH 约为 7.4 7.5）。若 pH 偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。0.2mol/L磷酸盐缓冲液（pH5.7 8.0）pH 0.2mol/L NaH2PO4(ml)0.2mol/L Na2HPO4(ml)5.7 93.5 6.5 5.8 92.0 8.0 5.9 90.0 10.0 6.0 87.7 12.3 6.1 85.0 15.0 6.2 81.5 18.5 6.3 77.5 22.5 6.4 73.5 26.5 6.5 68.5 3

6、1.5 6.6 62.5 37.5 6.7 56.5 43.5 文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7

7、M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6

8、Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK

9、8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5

10、P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2

11、D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:

12、CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P86.8 51.0 49.0 6.9 45.0 55.0 7.0 39.0 61.0 7.1 33.0 67.0 7.2 28.0 72.0 7.3 23.0 67.0 7.4 19.0 81.0 7.

13、5 16.0 84.0 7.6 13.0 87.0 7.7 10.5 89.5 7.8 8.5 91.5 7.9 7.0 93.0 8.0 5.3 94.7 0.05mol/L磷酸盐缓冲液(pH7.0)如何配制？文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2

14、ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档

15、编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M

16、3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q

17、2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8

18、文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K

19、8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A1

20、0Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4

21、P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K

22、7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1

23、A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8

24、T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A

25、7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7

26、M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6

27、Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK

28、8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5

29、P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2

30、D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:

31、CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 H

32、D5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 Z

33、W2D6Y8T4P8PH3-10 缓冲溶液的配制方法1磷酸氢二钠柠檬酸缓冲液pH 0.2mol/LNa2HPO4（毫升）0.1mol/L 柠檬酸（毫升）pH 0.2mol/LNa2HPO4（毫升）0.1mol/L 柠檬酸（毫升）2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 0.40 1.24 2.18 3.17 4.11 4.94 5.70 6.44 7.10 7.71 8.28 8.82 10.60 18.76 17.82 16.83 15.89 15.06 14.30 13.56 12.90 12.29 11.72 11.18 5.2 5.

34、4 5.6 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 10.72 11.15 11.60 12.09 12.63 13.22 13.85 14.55 15.45 16.47 17.39 18.17 9.28 8.85 8.40 7.91 7.37 6.78 6.15 5.45 4.55 3.53 2.61 1.83 文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编

35、码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3

36、 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2

37、 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文

38、档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8

39、M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10

40、Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P

41、8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P84.6 4.8 5.0 9.35 9.86 10.30 10.65 10.14 9.70 7.6 7.8 8.0 18.73 19.15 19.45 1.27 0.85 0.55 Na2HPO4分子量=14.98，0.2 mol/L 溶液为 28.40 克/升。Na2HPO42H2O 分子量=178.05，0.2 mol/L 溶液含 35.01 克/升。C4H2O7H2O 分子量=210.14，0.1 mol/L 溶液为 21.01 克/升。2柠檬酸氢氧化钠-盐酸缓冲液pH 钠离子浓度（mol/L）柠檬酸（克）

42、C6H8O7H2O 氢氧化钠(克)NaOH 97%盐酸（毫升）HCl（浓）最 终体积（升）2.2 3.1 3.3 4.3 5.3 5.8 6.5 0.20 0.20 0.20 0.20 0.35 0.45 0.38 210 210 210 210 245 285 266 84 83 83 83 144 186 156 160 116 106 45 68 105 126 10 10 10 10 10 10 10 使用时可以每升中加入1 克克酚，若最后 pH 值有变化，再用少量 50%氢氧化钠溶液或浓盐酸调节，冰箱保存。3 甘氨酸盐酸缓冲液（0.05mol/L）X 毫升 0.2 mol/L 甘氨酸

43、+Y 毫升 0.2 mol/L HCI，再加水稀释至200 毫升pH X Y pH X Y 2.0 2.4 2.6 2.8 50 50 50 50 44.0 32.4 24.2 16.8 3.0 3.2 3.4 3.6 50 50 50 50 11.4 8.2 6.4 5.0 甘氨酸分子量=75.07，0.2 mol/L 甘氨酸溶液含15.01 克/升。4邻苯二甲酸盐酸缓冲液（0.05 mol/L）X 毫升 0.2 mol/L 邻苯二甲酸氢钾+0.2 mol/L HCl，再加水稀释到20 毫升pH(20 )X Y pH(20)X Y 2.2 2.4 2.6 2.8 3.0 5 5 5 5 5

44、4.070 3.960 3.295 2.642 2.022 3.2 3.4 3.6 3.8 5 5 5 5 1.470 0.990 0.597 0.263 邻苯二甲酸氢钾分子量=204.23，0.2 mol/L 邻苯二甲酸氢溶液含40.85 克/升5柠檬酸柠檬酸钠缓冲液（0.1 mol/L）pH 0.1mol/L 柠檬酸（毫升）0.1 mol/L 柠檬酸钠（毫升）pH 0.1mol/L 柠檬酸（毫升）0.1mol/L 柠檬酸钠（毫升）文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P

45、8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7

46、K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A

47、10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T

48、4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7

49、K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M

50、1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y8T4P8文档编码:CK8A7K7K8M3 HD5P7M1A10Q2 ZW2D6Y