BD matrigel basement membrane matrix.pdf

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1、BD MatrigelBasementMembraneMatrixCertifiedLDEV-FreeBD Human Umbilical Vein Endothelial Cells(HUVEC-2)stained with Calcein AM and cultured onBD Matrigel Matrix.Confocal image(4x)taken using the BD Pathway BioImager.BD Matrigel Basement MembraneMatrix is a solubilized basementmembrane preparation extr

2、actedfrom the Engelbreth-Holm-Swarm(EHS)mouse sarcoma,a tumor rich inextracellular matrix proteins to includelaminin(a major component),collagenIV,heparan sulfate proteoglycans,andentactin/nidogen.1,2 BD Matrigel Matrixalso contains TGF-beta,epidermalgrowth factor,insulin-like growthfactor,fibroblas

3、t growth factor,tissueplasminogen activator,3,4 and othergrowth factors which occur naturally inthe EHS tumor.BD Matrigel Basement Membrane Matrix is effective for the attachment anddifferentiation of both normal and transformed anchorage dependent epithelioid and othercell types.These include neuro

4、ns,5,6 Sertoli cells,7 chick lens,8 and vascular endothelialcells,9 and hepatocytes.10 BD Matrigel Matrix will influence gene expression in adult rathepatocytes11 as well as three dimensional culture in mouse12-15 and human16,17 mammaryepithelial cells.It will support in vivo peripheral nerve regene

5、ration,18-20 can be used formetabolism and toxicology studies,21,22 and is the basis for several types of tumor cellinvasion assays.23,24 BD Matrigel Matrix provides the substrate necessary for the study ofangiogenesis both in vitro25,26 and in vivo.27-29 BD Matrigel Matrix also supports in vivoprop

6、agation of human tumors in immunosupressed mice.30-32Wide Selection of Basement Membrane Matrices:Wide Selection of Basement Membrane Matrices:BD Matrigel Matrix Growth FactorBD Matrigel Matrix Growth FactorReduced(GFR)Reduced(GFR)is suited for applicationswhere a more highly defined basementmembran

7、e preparation is desired.Availablein standard GFR,High Concentration(HC),and Phenol Red-Free formats.BD Matrigel Matrix High ConcentrationBD Matrigel Matrix High Concentrationis suited for in vivo applications wherea high protein concentration augmentsgrowth of tumors.The high proteinconcentration a

8、lso allows the BD MatrigelMatrix Plug to maintain its integrity aftersubcutaneous injection into mice.Availablein Standard,Growth Factor Reduced(GFR),and Phenol Red-Free formats.BD Matrigel Matrix Phenol Red-FreeBD Matrigel Matrix Phenol Red-Free isrecommended for assays which requirecolor detection

9、(i.e.fluorescence).BD Matrigel hESC-qualified MatrixBD Matrigel hESC-qualified Matrix hasbeen qualified as mTeSR1-compatible byStemCell Technologies,thus eliminatingthe need for time-consuming screening,in order to provide the reproducibilityand consistency essential for your humanembryonic stem(hES

10、)cell research.ThemTeSR1 formulation and BD MatrigelMatrix have been shown to be a successfulcombination for feeder-free maintenance ofdifferent WiCell hES cell lines for up to 20passages(mTeSR1,StemCell TechnologiesCat.No.05850).BDBBD MatrigelBasementMembraneMatrixTypical Applications:Typical Appli

11、cations:Cell Growth and DifferentiationBD Matrigel Matrix is especially suitedfor the culture of polarized cells,suchas epithelial cells.It promotes thedifferentiation of many cells types,including hepatocytes,neurons,mammaryepithelial,endothelial,and smooth musclecells.In Vivo Angiogenesis StudiesB

12、D Matrigel Matrix HC can be usedto assess in vivo angiogenic activity ofdifferent compounds by subcutaneousinjection into mice(BD Matrigel PlugAssay).The plugs are subsequentlyremoved and analyzed for the formationof blood vessels.Augmentation of Tumor Growth inNude MiceBD Matrigel Matrix HC has bee

13、n shownto promote successful transplantationof many human tumor cells,includingprostatic,breast,small-cell lung,colon,adrenal carcinomas,melanomas,andlymphoblastic leukemia cells.Also,it hasbeen found to increase tumor growth ratesin vivo.To place an order,please contact yourTo place an order,please

14、 contact yourlocal distributor or nearestlocal distributor or nearestBD Biosciences office.BD Biosciences office.For technical assistance,please contactFor technical assistance,please contactScientific Support at:Scientific Support at:For literature citations and otherBD Extracellular Matrix product

15、s,please visit .For research use only.Not intended for use in diagnostic ortherapeutic procedures.mTeSR is a trademark of WiCell Research Institute.BD,BD Logo,and all other trademarks are the property of Becton,Dickinson and Company.2008 BDXEUR4247-00Quality Control:Quality Control:Mouse colonies ar

16、e routinely screened for pathogens via Mouse Antibody Production(MAP)testing Extensive PCR testing is performed to screen for a number of pathogens,includingLDEV,to ensure strict control of raw materials used during the manufacturing process Tested and found negative for bacteria,fungi,and mycoplasm

17、a Protein concentrations are determined by Lowry method Endotoxin units are measured by Limulus Amoebocyte Lysate assay BD Matrigel Matrix gel stability is tested for a period of 14 days at 37C Biological activity is determined for each lot using a neurite outgrowth assay.Chickdorsal root ganglia ar

18、e plated on a 1.0 mm layer of BD Matrigel Matrix and mustgenerate positive neurite outgrowth response after 48 hours without addition of nervegrowth factorOrdering InformationOrdering InformationDescriptionDescriptionQty.Qty.Cat.No.Cat.No.BD MatrigelBD Matrigel Basement Membrane Matrix Basement Memb

19、rane MatrixBD Matrigel Matrix5 ml356234BD Matrigel Matrix10 ml354234BD Matrigel Matrix5 x 10 ml356235BD Matrigel Matrix High Concentration10 ml354248BD Matrigel Matrix Phenol Red-Free10 ml356237BD Matrigel Matrix High Concentration,Phenol Red-Free10 ml354262BD Matrigel Matrix Growth Factor Reduced5

20、ml356230BD Matrigel Matrix Growth Factor Reduced10 ml354230BD Matrigel Matrix Growth Factor Reduced,10 ml354263High ConcentrationBD Matrigel Matrix Growth Factor Reduced,Phenol Red-Free 10 ml356231BD Matrigel hESC-qualified Matrix5 ml354277Typical protein concentrations for BD Matrigel Matrix are be

21、tween 9-12 mg/ml.BD Matrigel MatrixHigh Concentration is 18-22 mg/ml.A lot-specific Certificate of Analysis is included in eachBD Matrigel Matrix shipment noting exact endotoxin and protein concentrations.BD BiosciencesBD BiosciencesEuropeEurope-Discovery LabwarePOB 13,Erembodegem-Dorp 86B-9320 Erem

22、bodegemTel.:(32)2 400 98 95Fax:(32)2 401 70 94contact_AUSTRIA Tel:(3)1 NORWAY Tel:(47)BELGIUM Tel:(32)53 720.550customer_service_PORTUGAL Tel:(351)21 421.93.30enzifarmaenzifarma.ptDENMARK Tel:(45)43 43.45.66bdcs_dk_SPAIN Tel:(34)90 227.17.27servicio_de_EASTERN EUROPE(EMA)Tel:(49)SWEDEN Tel:(46)8 FINLAND Tel:(358)9 8870 SWITZERLAND Tel:(41)61 FRANCE Tel:(33)4 THE NETHERLANDS Tel:(31)20 582.94.20customer_service_GERMANY Tel:(49)UK Tel:(44)1865 781688BDUK_CustomerSGREECE Tel.:(30)210 940.77.41ITALY Tel:(39)02 48.240.1ordini_