外泌体在肿瘤中的研究.pptx

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1、外泌体在肿瘤研究中的应用,癌细胞作为受体,经典文献：,1.DendriticcellexosomesdirectlykilltumorcellsandactivatenaturalkillercellsviaTNFsuperfamilyligands;2.Aberrantlowexpressionofp85instromalfibroblastspromotesbreastcancercellmetastasisthroughexosome-mediatedparacrineWnt10b;3.ExosomeTransferfromStromaltoBreastCancerCellsRegula

2、tesTherapyResistancePathways;4.MicroRNAsTransferfromHumanMacrophagestoHepato-CarcinomaCellsandInhibitProliferation;,癌细胞作为供体,例：,1.CancerExosomesPerformCell-IndependentMicroRNABiogenesisandPromoteTumorigenesis;2.ExosomesSecretedunderHypoxiaEnhanceInvasivenessandStemnessofProstateCancerCellsbyTargeting

3、AdherensJunctionMolecules;3.CancerExosomesTriggerFibroblasttoMyofibroblastDifferentiation;4.Exosomederivedfromepigallocatechingallatetreatedbreastcancercellssuppressestumorgrowthbyinhibitingtumor-associatedmacrophageinfiltrationandM2polarization;5.Humantumorvirusutilizesexosomesforintercellularcommu

4、nication;6.MurineMammaryCarcinomaExosomesPromoteTumorGrowthbySuppressionofNKCellFunction;,应用：Thenovelbiomarkers,例:,1.ClinicalImpactofSerumExosomalMicroRNA-21AsaClinicalBiomarkerinHumanEsophagealSquamousCellCarcinoma；2.Glypican-1identifiescancerexosomesanddetectsearlypancreaticcancer;3.Double-strande

5、dDNAinexosomesanovelbiomarkerincancerdetection;4.ExosomallongnoncodingRNACRNDE-hasanovelserum-basedbiomarkerfordiagnosisandprognosisofcolorectalcancer;,应用：Therapy,例:,1.ExosomesderivedfromIL-12-anchoredrenalcancercellsincreaseinductionofspecificantitumorresponseinvitro;2.SystemicallyInjectedExosomesT

6、argetedtoEGFRDeliverAntitumorMicroRNAtoBreastCancerCells;3.AphaseIstudyofdexosomeimmunotherapyinpatientswithadvancednon-smallcelllungcancer;4.Dendriticcell-derivedexosomesasmaintenanceimmunotherapyafterfirstlinechemotherapyinNSCLC;,思路：,1.测序(蛋白/mRNA/miRNA/lncRNA/circRNA)2.测序(蛋白/mRNA/miRNA/lncRNA/circ

7、RNA)+临床验证3.功能+测序(蛋白/mRNA/miRNA/lncRNA/circRNA)4.功能+简单信号通路5.功能+蛋白/mRNA/miRNA/lncRNA/circRNA+下游机制6.功能+蛋白/mRNA/miRNA/lncRNA/circRNA+上游调控7.功能+蛋白/mRNA/miRNA/lncRNA/circRNA+上游调控+下游机制8.创新性想法提出,例：,1.ProteomicAnalysisofExosome-likeVesiclesDerivedfromBreastCancerCells,ANTICANCERRESEARCH.2.ClinicalImpactofSeru

8、mExosomalMicroRNA-21AsaClinicalBiomarkerinHumanEsophagealSquamousCellCarcinoma,Cancer.3.Exosome-TransmittedlncARSRPromotesSunitinibResistanceinRenalCancerbyActingasaCompetingEndogenousRNA,Cacnercell.4.Pancreaticcancerexosomesinitiatepre-metastaticnicheformationintheliver,NATURECELLBIOLOGY；5.Tumourex

9、osomeintegrinsdetermineorganotropicmetastasis,Nature;,经典文献:126-year-old“seed-and-soil”hypothesis,FuturemetastaticsitesUptakeExosomes,Exosomesredirectmetastaticdistribution,Organ-specifictumourexosomesinteractwithResidentcells.,Exosomesredirectmetastaticdistribution,ExosomalITGsdetermineorganotropism

10、,ExosomalITGsasorganotropismbiomarkers,胰腺癌早期诊断：Glypican-1identifiescancerexosomesanddetectsearlypancreaticcancer,nature,2015,GPC1isaspecificmarkerofcancerexosomes,GPC11circulatingexosomesareacancerbiomarker.GPC11crExoscontainoncogenicKRASG12D.,GPC11crExosdetectearlypancreaticcancer,LevelsofcirculatingGPC11Exosomesinformpancreaticcancerresectionoutcome,Elisa,GPC11crExosdetectearlyPanINlesions.,谢谢！,