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1、Mainpaper,WANGYuchunLIUQing,Contents,2,Introduction,WANGMinming,Discussion,GENGQi,Introduction,1,MainPaper,2,Parietalcells(secreteHCl),Chiefcells(secretepepsinogen),Gastricmucosa(secretesgastrin),Fundus,Corpus,Antrum,王闵铭你会介绍如何培养到endoderm时期吗？,K.M.McCrackenetal.,Nature,2014,WNTandFGFwithoutBMPdrivemor
2、phogenesisofguttubestructures,Tae-HeeKimetal.,Development,2016,WNTandFGFwithoutBMPdrivemorphogenesisofguttubestructures,WNT,FGFandBMPcooperatetopromoteamid-hindgutfate,butWNTandFGFactwithoutBMPtodrivemorphogenesisofguttubestructures.,K.M.McCrackenetal.,Nature,2014,Retinoicacidsignalingactsforpattern
3、ingofforegut,K.M.McCrackenetal.,Nature,2014,Additionalretinoicacidsignalingspecifiesgastricantrumfate,EGFpromotehGOformation,K.M.McCrackenetal.,Nature,2014,Landmarks,However,Thefirsthumangastrictissuefullyderivedinvitro.Arobustsystemtostudyantrallineageallocationandhostmicrobeinteractionsinthestomac
4、h,Nofundusstructureemergedduringculture.Donotallowstudiesoffundicbiologyanddisease.,WhatandHowtodonext?,ButIknowalsothatstillmoreinterestingdiscoverieswillbemadethatIhavenottheimaginationtodescribe-andIamawaitingthem,fullofcuriosityandenthusiasm.-LInusPauling,Whatcausesfundusspecification?,Different
5、molecularmarkers,Differentgeneexpressionlevel,Pathwayregulation,TheembryonicfundusdomainisSox2+Gata4+Pdx1,Irx2,Irx3,Irx5arehighlyexpressedinfundus,Microarraydata,FundicandantraldomainsareestablishedbyE10.5,Meanwhile,differentWntsignalingactivitiesareobserved,ThecanonicalWntsignallingpathwaywasactive
6、intheproximalstomachwhileexhibitedlittleornoactivityinthedistalstomach,Whatregulatesfundusspecification?,Lossoffunctiontest,Gainoffunctiontest,Disturbanceofmolecularmarkerexpression,Reducedgeneexpressionlevel,Histologicandcytologicalchanges,Wntsignallingwasrequiredforestablishingthefundus,Disruption
7、ofWntsignalingleadtoectopicPdx1expressionlossoffundicidentity,DisruptionofWntsignalingreducetheexpressionofIrx2,Irx3andIrx5.,Wntsignallingwasrequiredforestablishingthefundus,Wntsignallingwasrequiredforestablishingthefundus,Atp4bPdx1Cdh1,ThestomachincKOembryoswasmalformedandreducedinsizeatE18.5.(also
8、withPdx1ectopicexpression),Wntsignallingwasrequiredforestablishingthefundus,Parietalcellswerecompletelyabsentin-catenin-deficientepithelium,StimulationofWntsignalingdirectsposteriorforegutepitheliumintothefundiclineage.,TheuseofWntsignalinginorganoidestablishing,StimulationofWntsignalingthroughday29stablizefundicgeneexpression,Discussion,3,
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